Heterologous Expression of the AtNPR1 Gene in Olive and Its Effects on Fungal Tolerance.
Identifieur interne : 000074 ( Main/Exploration ); précédent : 000073; suivant : 000075Heterologous Expression of the AtNPR1 Gene in Olive and Its Effects on Fungal Tolerance.
Auteurs : Isabel Narváez [Espagne] ; Clara Pliego Prieto [Espagne] ; Elena Palomo-Ríos [Espagne] ; Louis Fresta [Espagne] ; Rafael M. Jiménez-Díaz [Espagne] ; Jose L. Trapero-Casas [Espagne] ; Carlos Lopez-Herrera [Espagne] ; Juan M. Arjona-Lopez [Espagne] ; Jose A. Mercado [Espagne] ; Fernando Pliego-Alfaro [Espagne]Source :
- Frontiers in plant science [ 1664-462X ] ; 2020.
Abstract
The NPR1 gene encodes a key component of systemic acquired resistance (SAR) signaling mediated by salicylic acid (SA). Overexpression of NPR1 confers resistance to biotrophic and hemibiotrophic fungi in several plant species. The NPR1 gene has also been shown to be involved in the crosstalk between SAR signaling and the jasmonic acid-ethylene (JA/Et) pathway, which is involved in the response to necrotrophic fungi. The aim of this research was to generate transgenic olive plants expressing the NPR1 gene from Arabidopsis thaliana to evaluate their differential response to the hemibiotrophic fungus Verticillium dahliae and the necrotroph Rosellinia necatrix. Three transgenic lines expressing the AtNPR1 gene under the control of the constitutive promoter CaMV35S were obtained using an embryogenic line derived from a seed of cv. Picual. After maturation and germination of the transgenic somatic embryos, the plants were micropropagated and acclimated to ex vitro conditions. The level of AtNPR1 expression in the transgenic materials varied greatly among the different lines and was higher in the NPR1-780 line. The expression of AtNPR1 did not alter the growth of transgenic plants either in vitro or in the greenhouse. Different levels of transgene expression also did not affect basal endochitinase activity in the leaves, which was similar to that of control plants. Response to the hemibiotrophic pathogen V. dahliae varied with pathotype. All plants died by 50 days after inoculation with defoliating (D) pathotype V-138, but the response to non-defoliating (ND) strains differed by race: following inoculation with the V-1242 strain (ND, race 2), symptoms appeared after 44-55 days, with line NPR1-780 showing the lowest disease severity index. This line also showed good performance when inoculated with the V-1558 strain (ND, race 1), although the differences from the control were not statistically significant. In response to the necrotroph R. necatrix, all the transgenic lines showed a slight delay in disease development, with mean area under the disease progress curve (AUDPC) values 7-15% lower than that of the control.
DOI: 10.3389/fpls.2020.00308
PubMed: 32265961
PubMed Central: PMC7100536
Affiliations:
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type="region">Andalousie</region></placeName></affiliation></author><author><name sortKey="Pliego Prieto, Clara" sort="Pliego Prieto, Clara" uniqKey="Pliego Prieto C" first="Clara" last="Pliego Prieto">Clara Pliego Prieto</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Genómica y Biotecnología, Fruticultura Subtropical y Mediterránea (IFAPA), Unidad Asociada de I+D+i al CSIC, Málaga, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Genómica y Biotecnología, Fruticultura Subtropical y Mediterránea (IFAPA), Unidad Asociada de I+D+i al CSIC, Málaga</wicri:regionArea><wicri:noRegion>Málaga</wicri:noRegion></affiliation></author><author><name sortKey="Palomo Rios, Elena" sort="Palomo Rios, Elena" uniqKey="Palomo Rios E" first="Elena" last="Palomo-Ríos">Elena Palomo-Ríos</name><affiliation wicri:level="4"><nlm:affiliation>Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, Málaga, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, Málaga</wicri:regionArea><orgName type="university">Université de Malaga</orgName><placeName><settlement type="city">Malaga</settlement><region nuts="2" type="region">Andalousie</region></placeName></affiliation></author><author><name sortKey="Fresta, Louis" sort="Fresta, Louis" uniqKey="Fresta L" first="Louis" last="Fresta">Louis Fresta</name><affiliation wicri:level="4"><nlm:affiliation>Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, Málaga, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, Málaga</wicri:regionArea><orgName type="university">Université de Malaga</orgName><placeName><settlement type="city">Malaga</settlement><region nuts="2" type="region">Andalousie</region></placeName></affiliation></author><author><name sortKey="Jimenez Diaz, Rafael M" sort="Jimenez Diaz, Rafael M" uniqKey="Jimenez Diaz R" first="Rafael M" last="Jiménez-Díaz">Rafael M. Jiménez-Díaz</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Agronomía, College of Agriculture and Forestry (ETSIAM), Universidad de Córdoba, Campus de Excelencia Internacional Agroalimentario ceiA3, Córdoba, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Agronomía, College of Agriculture and Forestry (ETSIAM), Universidad de Córdoba, Campus de Excelencia Internacional Agroalimentario ceiA3, Córdoba</wicri:regionArea><wicri:noRegion>Córdoba</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario ceiA3, Córdoba, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario ceiA3, Córdoba</wicri:regionArea><wicri:noRegion>Córdoba</wicri:noRegion></affiliation></author><author><name sortKey="Trapero Casas, Jose L" sort="Trapero Casas, Jose L" uniqKey="Trapero Casas J" first="Jose L" last="Trapero-Casas">Jose L. Trapero-Casas</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario ceiA3, Córdoba, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario ceiA3, Córdoba</wicri:regionArea><wicri:noRegion>Córdoba</wicri:noRegion></affiliation></author><author><name sortKey="Lopez Herrera, Carlos" sort="Lopez Herrera, Carlos" uniqKey="Lopez Herrera C" first="Carlos" last="Lopez-Herrera">Carlos Lopez-Herrera</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario ceiA3, Córdoba, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario ceiA3, Córdoba</wicri:regionArea><wicri:noRegion>Córdoba</wicri:noRegion></affiliation></author><author><name sortKey="Arjona Lopez, Juan M" sort="Arjona Lopez, Juan M" uniqKey="Arjona Lopez J" first="Juan M" last="Arjona-Lopez">Juan M. Arjona-Lopez</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario ceiA3, Córdoba, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario ceiA3, Córdoba</wicri:regionArea><wicri:noRegion>Córdoba</wicri:noRegion></affiliation></author><author><name sortKey="Mercado, Jose A" sort="Mercado, Jose A" uniqKey="Mercado J" first="Jose A" last="Mercado">Jose A. Mercado</name><affiliation wicri:level="4"><nlm:affiliation>Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, Málaga, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, Málaga</wicri:regionArea><orgName type="university">Université de Malaga</orgName><placeName><settlement type="city">Malaga</settlement><region nuts="2" type="region">Andalousie</region></placeName></affiliation></author><author><name sortKey="Pliego Alfaro, Fernando" sort="Pliego Alfaro, Fernando" uniqKey="Pliego Alfaro F" first="Fernando" last="Pliego-Alfaro">Fernando Pliego-Alfaro</name><affiliation wicri:level="4"><nlm:affiliation>Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, Málaga, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, Málaga</wicri:regionArea><orgName type="university">Université de Malaga</orgName><placeName><settlement type="city">Malaga</settlement><region nuts="2" type="region">Andalousie</region></placeName></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The <i>NPR1</i> gene encodes a key component of systemic acquired resistance (SAR) signaling mediated by salicylic acid (SA). Overexpression of <i>NPR1</i> confers resistance to biotrophic and hemibiotrophic fungi in several plant species. The <i>NPR1</i> gene has also been shown to be involved in the crosstalk between SAR signaling and the jasmonic acid-ethylene (JA/Et) pathway, which is involved in the response to necrotrophic fungi. The aim of this research was to generate transgenic olive plants expressing the <i>NPR1</i> gene from <i>Arabidopsis thaliana</i> to evaluate their differential response to the hemibiotrophic fungus <i>Verticillium dahliae</i> and the necrotroph <i>Rosellinia necatrix</i>. Three transgenic lines expressing the <i>AtNPR1</i> gene under the control of the constitutive promoter CaMV35S were obtained using an embryogenic line derived from a seed of cv. Picual. After maturation and germination of the transgenic somatic embryos, the plants were micropropagated and acclimated to <i>ex vitro</i> conditions. The level of <i>AtNPR1</i> expression in the transgenic materials varied greatly among the different lines and was higher in the <i>NPR1</i>-780 line. The expression of <i>AtNPR1</i> did not alter the growth of transgenic plants either <i>in vitro</i> or in the greenhouse. Different levels of transgene expression also did not affect basal endochitinase activity in the leaves, which was similar to that of control plants. Response to the hemibiotrophic pathogen <i>V. dahliae</i> varied with pathotype. All plants died by 50 days after inoculation with defoliating (D) pathotype V-138, but the response to non-defoliating (ND) strains differed by race: following inoculation with the V-1242 strain (ND, race 2), symptoms appeared after 44-55 days, with line <i>NPR1</i>-780 showing the lowest disease severity index. This line also showed good performance when inoculated with the V-1558 strain (ND, race 1), although the differences from the control were not statistically significant. In response to the necrotroph <i>R. necatrix</i>, all the transgenic lines showed a slight delay in disease development, with mean area under the disease progress curve (AUDPC) values 7-15% lower than that of the control.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32265961</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Heterologous Expression of the <i>AtNPR1</i> Gene in Olive and Its Effects on Fungal Tolerance.</ArticleTitle><Pagination><MedlinePgn>308</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2020.00308</ELocationID><Abstract><AbstractText>The <i>NPR1</i> gene encodes a key component of systemic acquired resistance (SAR) signaling mediated by salicylic acid (SA). Overexpression of <i>NPR1</i> confers resistance to biotrophic and hemibiotrophic fungi in several plant species. The <i>NPR1</i> gene has also been shown to be involved in the crosstalk between SAR signaling and the jasmonic acid-ethylene (JA/Et) pathway, which is involved in the response to necrotrophic fungi. The aim of this research was to generate transgenic olive plants expressing the <i>NPR1</i> gene from <i>Arabidopsis thaliana</i> to evaluate their differential response to the hemibiotrophic fungus <i>Verticillium dahliae</i> and the necrotroph <i>Rosellinia necatrix</i>. Three transgenic lines expressing the <i>AtNPR1</i> gene under the control of the constitutive promoter CaMV35S were obtained using an embryogenic line derived from a seed of cv. Picual. After maturation and germination of the transgenic somatic embryos, the plants were micropropagated and acclimated to <i>ex vitro</i> conditions. The level of <i>AtNPR1</i> expression in the transgenic materials varied greatly among the different lines and was higher in the <i>NPR1</i>-780 line. The expression of <i>AtNPR1</i> did not alter the growth of transgenic plants either <i>in vitro</i> or in the greenhouse. Different levels of transgene expression also did not affect basal endochitinase activity in the leaves, which was similar to that of control plants. Response to the hemibiotrophic pathogen <i>V. dahliae</i> varied with pathotype. All plants died by 50 days after inoculation with defoliating (D) pathotype V-138, but the response to non-defoliating (ND) strains differed by race: following inoculation with the V-1242 strain (ND, race 2), symptoms appeared after 44-55 days, with line <i>NPR1</i>-780 showing the lowest disease severity index. This line also showed good performance when inoculated with the V-1558 strain (ND, race 1), although the differences from the control were not statistically significant. In response to the necrotroph <i>R. necatrix</i>, all the transgenic lines showed a slight delay in disease development, with mean area under the disease progress curve (AUDPC) values 7-15% lower than that of the control.</AbstractText><CopyrightInformation>Copyright © 2020 Narváez, Pliego Prieto, Palomo-Ríos, Fresta, Jiménez-Díaz, Trapero-Casas, Lopez-Herrera, Arjona-Lopez, Mercado and Pliego-Alfaro.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Narváez</LastName><ForeName>Isabel</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pliego Prieto</LastName><ForeName>Clara</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Departamento de Genómica y Biotecnología, Fruticultura Subtropical y Mediterránea (IFAPA), Unidad Asociada de I+D+i al CSIC, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Palomo-Ríos</LastName><ForeName>Elena</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fresta</LastName><ForeName>Louis</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiménez-Díaz</LastName><ForeName>Rafael M</ForeName><Initials>RM</Initials><AffiliationInfo><Affiliation>Departamento de Agronomía, College of Agriculture and Forestry (ETSIAM), Universidad de Córdoba, Campus de Excelencia Internacional Agroalimentario ceiA3, Córdoba, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario ceiA3, Córdoba, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Trapero-Casas</LastName><ForeName>Jose L</ForeName><Initials>JL</Initials><AffiliationInfo><Affiliation>Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario ceiA3, Córdoba, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lopez-Herrera</LastName><ForeName>Carlos</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario ceiA3, Córdoba, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arjona-Lopez</LastName><ForeName>Juan M</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario ceiA3, Córdoba, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mercado</LastName><ForeName>Jose A</ForeName><Initials>JA</Initials><AffiliationInfo><Affiliation>Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pliego-Alfaro</LastName><ForeName>Fernando</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>03</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Olea europaea</Keyword><Keyword MajorTopicYN="N">SAR response</Keyword><Keyword MajorTopicYN="N">Verticillium wilt</Keyword><Keyword MajorTopicYN="N">genetic transformation</Keyword><Keyword MajorTopicYN="N">soil-borne pathogens</Keyword><Keyword MajorTopicYN="N">white root rot</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate 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IdType="pubmed">17879991</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country><region><li>Andalousie</li></region><settlement><li>Malaga</li></settlement><orgName><li>Université de Malaga</li></orgName></list><tree><country name="Espagne"><region name="Andalousie"><name sortKey="Narvaez, Isabel" sort="Narvaez, Isabel" uniqKey="Narvaez I" first="Isabel" last="Narváez">Isabel Narváez</name></region><name sortKey="Arjona Lopez, Juan M" sort="Arjona Lopez, Juan M" uniqKey="Arjona Lopez J" first="Juan M" last="Arjona-Lopez">Juan M. Arjona-Lopez</name><name sortKey="Fresta, Louis" sort="Fresta, Louis" uniqKey="Fresta L" first="Louis" last="Fresta">Louis Fresta</name><name sortKey="Jimenez Diaz, Rafael M" sort="Jimenez Diaz, Rafael M" uniqKey="Jimenez Diaz R" first="Rafael M" last="Jiménez-Díaz">Rafael M. Jiménez-Díaz</name><name sortKey="Jimenez Diaz, Rafael M" sort="Jimenez Diaz, Rafael M" uniqKey="Jimenez Diaz R" first="Rafael M" last="Jiménez-Díaz">Rafael M. Jiménez-Díaz</name><name sortKey="Lopez Herrera, Carlos" sort="Lopez Herrera, Carlos" uniqKey="Lopez Herrera C" first="Carlos" last="Lopez-Herrera">Carlos Lopez-Herrera</name><name sortKey="Mercado, Jose A" sort="Mercado, Jose A" uniqKey="Mercado J" first="Jose A" last="Mercado">Jose A. Mercado</name><name sortKey="Palomo Rios, Elena" sort="Palomo Rios, Elena" uniqKey="Palomo Rios E" first="Elena" last="Palomo-Ríos">Elena Palomo-Ríos</name><name sortKey="Pliego Alfaro, Fernando" sort="Pliego Alfaro, Fernando" uniqKey="Pliego Alfaro F" first="Fernando" last="Pliego-Alfaro">Fernando Pliego-Alfaro</name><name sortKey="Pliego Prieto, Clara" sort="Pliego Prieto, Clara" uniqKey="Pliego Prieto C" first="Clara" last="Pliego Prieto">Clara Pliego Prieto</name><name sortKey="Trapero Casas, Jose L" sort="Trapero Casas, Jose L" uniqKey="Trapero Casas J" first="Jose L" last="Trapero-Casas">Jose L. Trapero-Casas</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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